解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Antagonism of the calcitonin gene-related peptide (CGRP) receptor may be a useful approach for migraine treatment. Selective PEGylated peptide antagonists to the CGRP receptor are described, derived from CGRP(8-37) with polymer derivatization at an engineered lysine-25 residue. Potent PEGylated peptides with improved ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22270
更新日期:2013-07-01 00:00:00
abstract::Deoxyribozyme and aptamer selections are typically conducted in aqueous buffer solutions. Using nonaqueous cosolvents in selection experiments will help expand the activity of deoxyribozymes with non-oligonucleotide substrates and will allow identification of new aptamers for nonprotein targets. We undertook in vitro ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22191
更新日期:2013-06-01 00:00:00
abstract::Inherent chemical programmability available in peptide-based hydrogels has allowed diversity in the development of these materials for use in biomedical applications. Within the 20 natural amino acids, a range of chemical moieties are present. Here we used a mixing-induced self-assembly of two oppositely charged pepti...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22194
更新日期:2013-04-01 00:00:00
abstract::The discovery of extreme halophile microorganisms in the Dead Sea, which are specifically dependent on a multimolar salt environment to survive, stimulated major developments in biology and physical chemistry. The minireview focuses on the molecular level. After a brief introduction to the history of halophile studies...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22154
更新日期:2013-04-01 00:00:00
abstract::Polymeric macromolecules, when viewed abstractly as strings of symbols, can be treated in terms of formal language theory, providing a mathematical foundation for characterizing such strings both as collections and in terms of their individual structures. In addition this approach offers a framework for analysis of ma...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22101
更新日期:2013-03-01 00:00:00
abstract::The post-translational modification of histones has significant effects on overall chromatin function. One such modification is citrullination, which is catalyzed by the protein arginine deiminases (PADs), a unique family of enzymes that catalyzes the hydrolysis of peptidyl-arginine to form peptidyl-citrulline on hist...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22127
更新日期:2013-02-01 00:00:00
abstract::The mechanisms of bridge-mediated electron transfer (ET) reactions vary from coherent deep tunneling to thermally activated hopping. This short review focuses on some developments in the study of protein ET mechanisms at the molecular and at the cellular levels. It explains experimental and theoretical work on the inf...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22169
更新日期:2013-01-01 00:00:00
abstract::Elastin-like polypeptide (ELP) fusions have been designed to allow large-scale, nonchromatographic purification of many soluble proteins by using the inverse transition cycling (ITC) method; however, the sensitivity of the aqueous lower critical solubility phase transition temperature (T(t)) of ELPs to the addition of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22137
更新日期:2013-01-01 00:00:00
abstract::Piscidin 1 (Pis-1) has a high broad-spectrum activity against bacteria, fungi, and viruses but it also has a moderate hemolytic activities. To improve the antibacterial activity and to reduce toxicity, mutants Pis-1AA (G8A/G13A double mutant) and Pis-1PG (G8P mutant) have been designed based on the crystal structure o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22116
更新日期:2012-12-01 00:00:00
abstract::A systematic molecular dynamics (MD) simulation has been carried out on collagen-like peptides with different combinations of interruptions in the Gly-X(AA) -Y(AA) repeats. Although experimental studies have been carried out to elucidate the structural consequences of homotrimeric collagen-like peptides, this is the f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22085
更新日期:2012-11-01 00:00:00
abstract::Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22077
更新日期:2012-10-01 00:00:00
abstract::A natural rubber was identified and characterized for the first time in the latex of the perennial Mediterranean shrub Euphorbia characias. Four different methods, i.e., acetone, acetic acid, trichloroacetic acid, and Triton® X-100, followed by successive treatments with cyclohexane/ethanol, were employed to extract t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22044
更新日期:2012-08-01 00:00:00
abstract::We prepared the water soluble model peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG, to throw light on the local structure of spidroin 1 (MaSpl) protein in spider dragline silk of Nephila clavipes before and after spinning. Solution (13) C NMR showed that the conformation of the peptide in aqueous solution was essentially ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21718
更新日期:2012-06-01 00:00:00
abstract::In this article, we review current knowledge about the silk produced by the larvae of bees, ants, and hornets [Apoidea and Vespoidea: Hymenoptera]. Different species use the silk either alone or in composites for a variety of purposes including mechanical reinforcement, thermal regulation, or humidification. The chara...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21702
更新日期:2012-06-01 00:00:00
abstract::The X-ray diffraction analysis of a stereocontrolled heterochiral designed model peptide Boc-(D) Pro-Thr-OMe (1) revealed the existence of an unusual folded molecular structure, stabilized via an effective unconventional C---H…O type intramolecular hydrogen-bond, encompassing a noncovalent 12-membered ring-motif. Toge...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21705
更新日期:2012-01-01 00:00:00
abstract::Amphipathic peptides composed of alternating polar and nonpolar residues have a strong tendency to self-assemble into one-dimensional, amyloid-like fibril structures. Fibrils derived from peptides of general (XZXZ)(n) sequence in which X is hydrophobic and Z is hydrophilic adopt a putative β-sheet bilayer. The bilayer...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22058
更新日期:2012-01-01 00:00:00
abstract::Caspases comprise a family of dimeric cysteine proteases that control apoptotic programmed cell death and are therefore critical in both organismal development and disease. Specific inhibition of individual caspases has been repeatedly attempted, but has not yet been attained. Caspase-9 is an upstream or initiator cas...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22080
更新日期:2012-01-01 00:00:00
abstract::While our understanding of the folding and structure of water-soluble proteins has progressed to the point where they can be artificially designed and produced from first principles, there has been only limited work toward the de novo design of membrane proteins. Such studies have been hindered in large part due to th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22149
更新日期:2012-01-01 00:00:00
abstract::Per-O-methylated β-cyclodextrin (CD) bearing an iodoacetamide group at the 6-position was synthesized to functionalize protein surfaces. Bovine serum albumin (BSA) was quantitatively modified with the CD derivative by the S(N) 2 reaction of iodoacetamide with a cysteine residue (Cys34) on the BSA surface. The resultan...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21695
更新日期:2012-01-01 00:00:00
abstract::The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21713
更新日期:2012-01-01 00:00:00
abstract::Host defense peptides are conserved components of innate immune response present among all classes of life. These peptides are potent, broad spectrum antimicrobial agents with potential as novel therapeutic compounds. Also, the ability of host defense peptides to modulate immunity is an emerging therapeutic concept si...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22076
更新日期:2012-01-01 00:00:00
abstract::The study of biologically active peptides is critical to the understanding of physiological pathways, especially those involved in the development of disease. Historically, the measurement of biologically active endogenous peptides has been undertaken by radioimmunoassay, a highly sensitive and robust technique that p...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22109
更新日期:2012-01-01 00:00:00
abstract::Amyloid aggregates have been implicated in the pathogenesis of diseases such as type 2 diabetes, Alzheimer's, Parkinson's, and prion disease. Recently determined microcrystal structures of several short peptide segments derived from fibril-forming proteins revealed coexistence of alternative aggregation modes (amyloid...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21731
更新日期:2012-01-01 00:00:00
abstract::The occurrence of a highly conserved 11-mer repeating motif in the primary sequence is a major characteristic of group 3 late embryogenesis abundant (LEA3) proteins, which are strongly associated with abiotic stress tolerance of the plants. In this study, the three-dimensional structure, mimetic membrane association, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21693
更新日期:2012-01-01 00:00:00
abstract::For systems involving highly and oppositely charged proteins, electrostatic forces dominate association and contribute to biomolecular complex stability. Using experimental or theoretical alanine-scanning mutagenesis, it is possible to elucidate the contribution of individual ionizable amino acids to protein associati...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21644
更新日期:2011-11-01 00:00:00
abstract::It is becoming increasingly clear that characterization of the protein ensemble-the collection of all conformations of which the protein is capable-will be a critical step in developing a full understanding of the linkage between structure, dynamics, and function. X-ray solution scattering in the small angle (SAXS) an...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21631
更新日期:2011-08-01 00:00:00
abstract::GSK3β plays an important role in many physiological functions; dysregulated GSK3β is involved in human diseases such as diabetes, cancer, and Alzheimer's disease. This study uses MD simulations to determine the interaction between GSK3β and a peptide derived from GSKIP, a novel GSK3β interacting protein. Results show ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21603
更新日期:2011-07-01 00:00:00
abstract::The Lifson-Roig Model (LRM) and all its variants describe the α-helix to coil transition in terms of additive component-free energies within a free energy decomposition scheme, and these contributions are interpreted through sequence-context dependent nucleation and propagation parameters. Although this phenomenologic...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21572
更新日期:2011-04-01 00:00:00
abstract::The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21561
更新日期:2011-03-01 00:00:00
abstract::Elucidation of mechanisms underlying collagen fibril assembly and matrix-induced guidance of cell fate will contribute to the design and expanded use of this biopolymer for research and clinical applications. Here, we define how Type I collagen oligomers affect in-vitro polymerization kinetics as well as fibril micros...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21537
更新日期:2011-02-01 00:00:00
abstract::Structural characterization of G protein-coupled receptors (GPCRs) is hindered by the inherent hydrophobicity, flexibility, and large size of these signaling proteins. Insights into conformational preferences and the three-dimensional (3D) structure of domains of these receptors can be obtained using polypeptide fragm...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21614
更新日期:2011-01-01 00:00:00
abstract::Designed ligands that inhibit protein-protein interactions involved in gene expression are valuable as reagents for genomics research and as leads for drug discovery efforts. Selective modulation of protein-protein interactions has proven to be a daunting task for synthetic ligands; however, the last decade has seen s...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21546
更新日期:2011-01-01 00:00:00
abstract::Four members of the carcinoembryonic antigen family, CEACAMs 1, 3, 6, and 8, are expressed on human neutrophils and can trigger an activation signal that increases neutrophil adhesion to human umbilical vein endothelial cell (HUVEC) monolayers. To identify active sites on CEACAM1, we previously performed molecular mod...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21447
更新日期:2011-01-01 00:00:00
abstract::Peptoids, oligomers of N-substituted glycine, have been valuable targets for study and diverse application as peptidomimetics and as nanomaterials. Their conformational heterogeneity has made the study of peptoid structures using high-resolution analyses challenging, limiting our understanding of the physiochemical fe...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21605
更新日期:2011-01-01 00:00:00
abstract::The 83-residue conopeptide (p21a) and its corresponding nonhydroxylated analog were isolated from the injected venom of Conus purpurascens. The complete conopeptide sequences were derived from Edman degradation sequencing of fragments from tryptic, chymotryptic and cyanogen bromide digestions, p21a has a unique, 10-cy...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21406
更新日期:2011-01-01 00:00:00
abstract::Core peptide is a hydrophobic peptide derived from the T-cell antigen receptor-alpha chain (TCR-alpha) transmembrane region with therapeutic potential. The mechanism by which the peptide inserts into the membrane, including any requirements to change conformational or association states during the insertion, is unclea...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21423
更新日期:2011-01-01 00:00:00
abstract::Nodal, a member of the transforming growth factor-β superfamily, is a potent embryonic morphogen also implicated in tumor progression. Up to date structural information on the interaction of Nodal with its molecular partners are unknown. To deepen our understanding about mechanisms underlying both embryonic developmen...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21517
更新日期:2010-11-01 00:00:00
abstract::To validate the existing hypothesis put forward by Navarro et al., we performed single crystal X-ray diffraction structural analysis of a designed model peptide incorporating an unsubstituted achiral γ-aminobutyric acid: Boc-Pro-γ-Abu-OH (1) lacking C-terminal amide group. The analysis established existence of an over...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21511
更新日期:2010-11-01 00:00:00
abstract::Formation of toxic amyloid structures is believed to be associated with various late-onset neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. The fact that many proteins in addition to those that are associated with clinical conditions have the potential to form amyloid fibrils in vitro provides...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21477
更新日期:2010-10-01 00:00:00
abstract::Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21482
更新日期:2010-09-01 00:00:00